Customer-friendly solutions

RNA sequencing (RNA-seq) using NGS enables the identification and quantification of gene expression in biological samples. DNASense offers RNA-seq of individual prokaryotic and eukaryotic organisms (transcriptomics) as well as mixed microbial communities (metatranscriptomics). We provide full sample-to-answer RNA-seq solutions with cutting-edge bioinformatic pipelines for identification of differentially expressed genes (DEGs). Our workflows support mapping and identification of RNA transcripts by either de novo assembly, reference genomes or purpose built reference-hybrids (please refer to our genomics or metagenomics workflows for more information).

A crucial prerequisite for useful RNA-seq results is a suitably scaled experiment design. The number of biological replicates and required sequencing depths are highly dependent on the experiment’s scientific purpose, sample complexity and total transcript content. For large-scale projects setting up a pilot study is always the recommendation.

Do you also need a genome or metagenome reference for your transcriptomics or metatranscriptomics experiment? The team behind DNASense has extensive experience within the fields of genomics and metagenomics, and our active involvement in state-of-the-art methods and sequencing platforms (read about it in Nature Methods) ensures that customers obtain valuable insight from our tailored bioinformatic analyses.

The table below provides general recommendations, but if you are considering to do (meta)transcriptomics, we encourage you to contact DNASense already during the experimental planning and design, setting the foundation for the most optimal result outcome for your project.

Our standard package includes: Optional pre- and post-project meeting with a DNASense specialist, RNA extraction, rRNA depletion, library preparation and cDNA sequencing, Differential Gene Expression (DGE) analysis (mapping/de novo assembly, gene annotation, quantification and statistical analysis), online-access to raw data and result files, a detailed project report with ready to use for publication materials and methods and publication grade illustrations.

Add-on services (non-exhaustive list): functional annotation (KO, GO), functional enrichment analysis, LEfSe analysis, manual curation of metabolic pathways, Data submission (e.g. ENA).

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