Transcriptomics

Transcriptomics is the study of cellular gene expression, and it is often used to detect activity changes prompted by environmental stimuli. The benefit of doing sequencing based transcriptomics is the unbiased detection of novel transcripts, high specificity and easily scalable sensitivity and high throughput. Transcriptomics is usually performed on pure or simple cultures as good reference genomes are needed, but can in theory be done on complex samples as well. At DNASense we have a lot of experience with transcriptomics of pure cultures from both environmental and clinical contexts as well as studies of more complex communities from enrichment bioreactors.

  • RNA extraction
  • Depletion of ribosomal RNA
  • Illumina sequencing library preparation
  • Illumina MiSeq sequencing (150 bp)
  • > 1,000,000 million reads/sample (Suited for pure culture)
  • Sequencing QC report
  • Mapping to reference genome
  • Analysis of differential expression
Pricing:
From 700 EUR/sample
(minimum of 6 samples)

Add-on services:
Custom RNA purification, extra sequence reads, data submission to online databases, pathway enrichment analysis etc.